Niedersächsische Staats- und Universitätsbibliothek Göttingen, Germany http://webdoc.sub.gwdg.de/diss/2005/papoucheva/index.html http://gso.gbv.de/DB=2.1/CMD?ACT=SRCHA&IKT=12&SRT=YOP&TRM=487798775 eng 2004 Papoucheva, Ekaterina The Functional Role of Palmitoylation of the 5-HT 1A receptor Elektronische Ressourcen im Fernzugriff Zellbiologie In the present study, we verified that the mouse 5-hydroxytryptamine(1A) (5-HT(1A)) receptor is modified by palmitic acid, which is covalently attached to the protein through a thioester-type bond. Palmitoylation efficiency was not modulated by receptor stimulation with agonists. Block of protein synthesis by cycloheximide resulted in a significant reduction of receptor acylation, suggesting that palmitoylation occurs early after synthesis of the 5-HT(1A) receptor. Furthermore, pulse-chase experiments demonstrated that fatty acids are stably attached to the receptor. Two conserved cysteine residues 417 and 420 located in the proximal C-terminal domain were identified as acylation sites by site-directed mutagenesis. To address the functional role of 5-HT(1A) receptor acylation, we have analyzed the ability of acylation-deficient mutants to interact with heterotrimeric G(i) protein and to modulate downstream effectors 3-4,6,11-12 0007:31-05-05 0007:31-05-05 14:44:05.000 0007:31-05-05 0 0 Oau 487798775 1 html http://webdoc.sub.gwdg.de/diss/2005/papoucheva/index.html eng eng 2004 ho Elektronische Ressource XA-DE-NI The Functional Role of Palmitoylation of the 5-HT 1A receptor Die Funktionelle Rolle der Palmitilierung des 5-HT 1A Rezeptor vorgelegt von Ekaterina Papoucheva Die Funktionelle Rolle der Palmitilierung des 5-HT 1A Rezeptor Ekaterina Papoucheva Göttingen Niedersächsische Staats- und Universitätsbibliothek Online Resource (117S., 1.380 KB) Ill., graph. Darst @Göttingen Univ., Diss., 2004 106421433 42.15 Zellbiologie In the present study, we verified that the mouse 5-hydroxytryptamine(1A) (5-HT(1A)) receptor is modified by palmitic acid, which is covalently attached to the protein through a thioester-type bond. Palmitoylation efficiency was not modulated by receptor stimulation with agonists. Block of protein synthesis by cycloheximide resulted in a significant reduction of receptor acylation, suggesting that palmitoylation occurs early after synthesis of the 5-HT(1A) receptor. Furthermore, pulse-chase experiments demonstrated that fatty acids are stably attached to the receptor. Two conserved cysteine residues 417 and 420 located in the proximal C-terminal domain were identified as acylation sites by site-directed mutagenesis. To address the functional role of 5-HT(1A) receptor acylation, we have analyzed the ability of acylation-deficient mutants to interact with heterotrimeric G(i) protein and to modulate downstream effectors 40 31-05-05 14:44:05.000 31-05-05 1383 0007 WH WF Ausleihbar| 0 722152981 mon 1 Ausleihbar 31-05-05 14:45:41.000 31-05-05 1383 0007 0 722152981 31-05-05 x ps 00 1>html http://webdoc.sub.gwdg.de/diss/2005/papoucheva/index.html urn:nbn:de:gbv:7-webdoc-102 1 2006-02-03T13:18:49 2006-02-03T13:18:49 KOPAL DIAS 2 urn:diasid:fty:kopal:0000000000000000000000 binary 2006-02-03T13:18:52+01:00 HTML-hul HTML Well-Formed, but not valid HTMLMetadata OtherLanguages ENG GER Title SUB Göttingen - Dissertationen - Papoucheva, Ekaterina: Die Funktionelle Rolle der Palmitilierung des 5-HT 1A Rezeptor MetaTags Meta Name DC.Creator.PersonalName Content Papoucheva, Ekaterina Meta Name DC.Creator.PersonalName.Address Content papusheva@yahoo.com Meta Name DC.Creator.PersonalName.DateOfBirth Content 1977-10-24 Meta Name DC.Creator.PersonalName.PlaceOfBirth Content Irkutsk, USSR Meta Name DC.Title Content Die Funktionelle Rolle der Palmitilierung des 5-HT 1A Rezeptor Meta Name DC.Title.Translated Content The Functional Role of Palmitoylation of the 5-HT 1A receptor Meta Name DC.Subject.Topic Content Mathematics and Natural Science Meta Name DC.Type Content Text.PhDThesis Meta Name DC.Language Content ENG Meta Name DC.Format Content application/pdf Meta Name DC.Date.Accepted Content 20041103 Meta Name DC.Contributor.Advisor Content Ponimaskin Evgeni, PD Dr Meta Name DC.Contributor.Referee Content Richter Diethelm Prof. Dr Meta Name DC.Contributor.Referee Content Jahn Reinhard Prof Dr Meta Name DC.Contributor.Referee Content Schürmann Friedrich-Wilhelm Prof Dr Meta Name DC.Subject Content G-protein-coupled receptor, palmitoylation, serotonin Meta Name DC.Subject Content G-proteine-gekoppelte Rezeptor, Palmitoylierung, Serotonin Meta Name DC.Subject Content 570 Biowissenschaften, Biologie Meta Name DC.Subject Content 42.15 Meta Name DC.Subject Content WH, WF Meta Name DC.Description Content #Abstract-ENG Meta Name DC.Description Content #Abstract-GER Meta Name DC.Rights Content http://webdoc.sub.gwdg.de/diss/copyr_diss.html Meta Name DC.Date.Created Content 2005-04-08 Meta Name DC.Description.Note Content Dissertation zur Erlangung des Doktortitels, angenommen von: Georg-August-Universität Göttingen, Meta Name DC.Description.Note Content Doctoral Dissertation accepted by: Georg-August-University of Göttingen, Meta Name DC.Publisher.CorporateName Content Niedersächsische Staats- und Universitätsbibliothek Göttingen Meta Name DC.Publisher.CorporateName.Address Content 37070 Göttingen Meta Name DC.Contributor.CorporateName Content Mathematisch-naturwissenschaftliche Fakultäten, Georg-August-Universität Göttingen Meta Name DC.Contributor.CorporateName.Address Content Mathematisch-naturwissenschaftliche Fakultäten, Georg-August-Universität Göttingen, 37073 Göttingen Meta Name 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